2025

Direct delivery of immune modulators to tumor-inflitrating lymphocytes using engineered extracellular vesicles.
Lyu X, Yamano T, Nagamori K, Imai S, Le TV, Bolidong D, Ueda M, Warashina S, Mukai H, Hayashi S, Matoba K, Nishino T, Hanayama R.
J Extracell Vesicles. in press (2025) link

Neurodegenerative disorder and fine particululate matter.
Kawahara H, Hanayama R.
Extracellular Fine Particles. Baba Y, Hanayama R, Akita H, Yasui T. eds. (Springer) in press (2025)


2024

Fibrinogen induces inflammatory responses via the immune activating receptor LILRA2.
Li Y, Hirayasu K, Hasegawa G, Tomita Y, Hashikawa Y, Hiwa R, Wang J, Furukawa A, Maenaka K, Arase H, Hanayama R.
Front Immunol. 15:1435236 (2024) link

The Rubicon-WIPI axis regulates exosome biogenesis during aging.
Yanagawa K, Kuma A, Hamasaki M, Kita S, yamamuro T, Nishino K, Nakamura S, Omori H, Kaminishi T, Oikawa S, Kato Y, Edahiro R, Kawagoe R, Taniguchi T, Tanaka Y, Shima T, Tabata K, Iwatani M, Bekku N, Hanayama R, Okada Y, Akimoto T, Kosako H, Takahashi A, Shimomura I, Sakata Y, Yoshimori T
Nat Cell Biol. 26:1558-70 (2024) link

Hematopoietic regeneration by HLA-A*0206-deficient clones in severe aplastic anemia without definitive immunosuppressive treatment.
Zaimoku Y, Sakai K, Tsuji N, Hosomichi K, Yamada S, Tran D, Kobayashi M, Sugiyama A, Hirayasu K, Mizumaki H, Ishiyama K, Hanayama R, Tomiyama Y, Nakao S.
Br J Haematol. Online ahead of print (2024) link

Quality and safety considerations for therapeutic products based on extracellular vesicles.
Takakura Y*, Hanayama R* (*co-corresponding authors), Akiyoshi A, Futaki S, Hida K, Ichiki T, Ishii-Watabe A, Kuroda M, Maki M, Miura Y, Okada Y, Seo N, Takeuchi T, Yamaguchi T, Yoshioka Y.
Pharm Res. 41(8):1573-94 (2024) link

Identification of the hybid gene LILRB5-3 by long-read sequencing and implication of its novel signalling function.
Hirayasu K, Khor S, Kawai Y, Shimada M, Omae Y, Hasegawa G, Hashikawa Y, Tanimoto H, Ohashi J, Hosomichi K, Tajima A, Nakamura H, Nakamura M, Tokunaga K, Hanayama R, Nagasaki M.
Front Immunol. 15:1398935 (2024) link

MHC class I-dressing is mediated via phosphatidylserineand recognition and is enhanced by poly I:C.
Hori A, Toyoura S, Fujiwara M, Taniguchi R, Yamano T, Hanayama R, Nakayama M.
iScience. 27(5):109704 (2024) link

Precise analysis of single small extracellular vesicles using flow cytometry.
Kobayashi H, Shiba T, Yoshida T, Bolidong D, Kato K, Mochizuki M, Seto T, Kawashiri S, Hanayama R.
Sci Rep. 14:7465 (2024) link

Extracellular vesicles promote silica nanoparticle aggregation that inhibits silica-induced cytotoxicity.
Yoshida T, Goto K, Kodama A, Bolidong D, Seto T, Hanayama R.
Arch Biochem Biophys. 755:109964 (2024) link

Peritumoral SPARC expression induced by exosomes from nasopharyngeal carcinoma infected Epstein-Barr Virus: a poor prognostic marker.
Dochi H, Kondo S, Komura S, Moriyama-Kita M, Komori T, Nanbo A, Sakaguchi M, Fukuyo M, Hamabe-Horiike T, Tanaka M, Mizokami H, Kano M, Kitagawa Y, Kobayashi E, Hirai N, Ueno T, Nakanishi Y, Endo K, Sugimoto H, Hanayama R, Kaneda A, Yoshizaki T.
Int J Cancer. 154(5):895-911 (2024) link


2023

Nanopipette Fabrication Guidelines for SICM Nanoscale Imaging.
Takahashi Y, Sasaki Y, Yoshida T, Honda K, Zhou Y, Miyamoto T, Motoo T, Higashi H, Shevchuk A, Korchev Y, Ida H, Hanayama R, Fukuma T.
Anal Chem. 95(34):12664-72 (2023) link

A validation study for the utility of serum microRNA as a diagnostic and prognostic marker in patients with osteosarcoma.
Araki Y, Asano N, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Abe K, Taniguchi Y, Yonezawa H, Morinaga S, Asano Y, Yoshida T, Hanayama R, Matsuzaki J, Ochiya T, Kawai A, Tsuchiya H.
Oncol Lett. 25(6):222 (2023) link

Preventing SARS-CoV-2 infection using anti-spike nanobody-IFN beta conjugated exosomes.
Lyu X, Imai S, Yamano T, Hanayama R.
Pharm Res. 40:927-35 (2023) link

Nanoscopic assessment of anti-SARS-CoV-2 spike neutralizing antibody using high-speed AFM.
Lim K*, Nishide G, Sajidah ES, Yamano T, Qiu Y, Yoshida T, Kobayashi A, Hazawa M, Ando T, Hanayama R* (*co-corresponding authors), Wong RW*.
Nano Lett. 23(2):619-28 (2023) link

EVs and Communication.
Yang X, Yoshida T, Hanayama R.
Encyclo Cell Biol. Bradshaw R, Hart G, Stahl P. eds. (Elsevier) 2:390-400 (2023) link


2022

Spatiotemporal tracking of small extracellular vesicle nanotopology in response to physicochemical stresses revealed by HS-AFM.
Sajidah ES, Lim K, Yamano T, Nishide G, Qiu Y, Yoshida T, Wang H, Kobayashi A, Hazawa M, Dewi FRP, Hanayama R, Ando T, Wong RW.
J Extracell Vesicles. 11(11):e12275 (2022) link

TIM4-affinity methods for purification or detection of extracellular vesicles targeting phosphatidylserine.
Yoshida T, Hanayama R.
Methods Mol Biol. 2466:23-36 (2022) link

Extracellular vesicles contribute to the metabolism of transthyretin amyloid in hereditary transthyretin amyloidosis.
Yamaguchi H, Kawahara H, Kodera N, Kumaki A, Tada Y, Tang Z, Sakai K, Ono K, Yamada M, Hanayama R.
Front Mol Biosci. 9:839917 (2022) link


2021

Millisecond dynamic of SARS-CoV-2 spike and its interaction with ACE2 receptor and small extracellular vesicles.
Lim KS, Nishide G, Yoshida T, Nakayama T, Kobayashi A, Hazama M, Hanayama R, Ando T, Wong RW.
J Extracell Vesicles. 10(14):e12170 (2021) link

Identication of small compounds regulating the secretion of extracellular vesicles via a TIM4-affinity ELISA.
Ma Y, Yoshida T, Matoba K, Kida K, Shintani R, Piao Y, Jin J, Nishino T, Hanayama R.
Sci Rep. 11(1):13471 (2021) link

Osteosarcoma-derived small extracellular vesicles enhance tumor metastasis and suppress osteoclastogenesis by miR-146a-5p.
Araki Y, Aiba H, Yoshida T, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Nguyen TD, Ishii K, Nojima T, Takahashi S, Murakami H, Tsuchiya H, Hanayama R.
Front Oncol. 11:667109 (2021) link

Structural and mechanical characteristics of exosomes from osteosarcoma cells explored by 3D-atomic force microscopy.
Yurtsever A, Yoshida T, Behjat A, Araki Y, Hanayama R, Fukuma T.
Nanoscale. 13(13):6661-77 (2021) link

Cytoplasmic DNA accumulation preferentially triggers cell death of myeloid leukemia cells by interacting with intracellular DNA sensing pathway.
Baba T, Yoshida T, Tanabe Y, Nishimura T, Morishita S, Gotoh N, Hirao A, Hanayama R, Mukaida N.
Cell Death Dis. 12(4):322 (2021) link

Characterization of LILRB3 and LILRA6 allelic variants in the Japanese population.
Hirayasu K, Sun J, Hasegawa G, Hashikawa Y, Hosomichi K, Tajima A, Tokunaga K, Ohashi J, Hanayama R.
J Hum Genet. 66(7):739-48 (2021) link

Tumor-secreted proliferin-1 regulates adipogenesis and lipolysis in cachexia.
Nguyen TD, Miyatake Y, Yoshida T, Kawahara H, Hanayama R.
Int J Cancer. 148(8):1982-92 (2021) link

Emerging roles of extracellular vesicles in physiology and disease.
Hanayama R.
J Biochem. 169(2):135-8 (2021) link


2020

Transient IGF-1R inhibition combined with osimertinib eradicates AXL-low expressing EGFR mutated lung cancers.
Wang R, Yamada T , Kita K, Taniguchi H, Arai S, Fukuda K, Terashima M, Ishimura A, Nishiyama A, Tanimoto A, Takeuchi S, Ohtsubo K, Yamashita K, Yamano T, Yoshimura A, Takayama K, Kaira K, Taniguchi Y, Atagi S, Uehara H, Hanayama R, Matsumoto I, Han X, Matsumoto K, Wang W, Suzuki T, Yano S.
Nat Commun. 11(1):4607 (2020) link

High-speed AFM reveals molecular dynamic of human influenza A hemagglutinin and its interaction with exosomes.
Lim KS, Kodera N, Wang H, Mohamed MS, Hazawa M, Kobayashi A, Yoshida T, Hanayama R, Yano S, Ando T, Wong RW.
Nano Lett. 20(9):6320-8 (2020) link

Glioma-derived extracellular vesicles promote tumor progression by conveying WT1.
Tsutsui T, Kawahara H, Kimura R, Dong Y, Jiapaer S, Sabit H, Zhang J, Yoshida T, Nakada M, Hanayama R.
Carcinogenesis. 41(9):1238-45 (2020) link

Novel properties of myoferlin in glucose metabolism via pathways involving modulation of adipose functions.
Nozato Y, Takami Y, Yamamoto K, Nagasawa M, Nozato S, Imaizumi Y, Takeshita H, Cheng W, Ito Y, Takeda S, Takeya Y, Sugimoto K, Nakagami H, Hanayama R, Rakugi H.
FASEB J. 34(2):2792-811 (2020) link


2019

Serum milk fat globule-EGF factor 8 (MFG-E8) as a diagnostic and prognostic biomarker in patients with hepatocellular carcinoma.
Shimagaki T, Yoshio S, Kawai H, Sakamoto Y, Doi H, Matsuda M, Mori T, Osawa Y, Fukai M, Yoshida T, Ma Y, Akita T, Tanaka J, Taketomi A, Hanayama R, Yoshizumi T, Mori M, Kanto T.
Sci Rep. 9(1):15788 (2019) link

Development of a method that delivers drugs to enveloped viruses.
Bahrini I, Hanayama R.
Biol Pharm Bull. 42(6):977-81 (2019) link

Aire-expressing ILC3-like cells in the lymph node display potent APC features.
Yamano T, Dobes J, Voboril M, Steinert M, Brabec T, Zietara N, Dobesova M, Ohnmacht C, Laan M, Peterson P, Benes V, Sedlacek R, Hanayama R, Kolar M, Klein L, Filipp D.
J Exp Med. 216(5):1027-37 (2019) link

Function and immunogenicity of gene-corrected iPSC-derived hepatocyte-like cells in restoring low density lipoprotein uptake in homozygous familial hypercholesterolemia.
Okada H, Nakanishi C, Yoshida S, Shimojima M, Yokawa J, Mori M, Tada H, Yoshimuta T, Hayashi K, Yamano T, Hanayama R, Yamagishi M, Kawashiri MA.
Sci Rep. 9(1):4695 (2019) link


2018

Myoferlin-mediated lysosomal exocytosis regulates cytotoxicity by phagocytes.
Miyatake Y, Yamano T, Hanayama R.
J Immunol. 201(10):3051-7 (2018) link

The role of exosome/extracellular vesicles in neural signal transduction.
Kawahara H, Hanayama R.
Biol Pharm Bull. 41(8):1119-25 (2018) link

Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release.
Obata Y, Kita S, Koyama Y, Fukuda S, Takeda H, Takahashi M, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nakamura Y, Nishizawa H, Funahashi T, Ranscht B, Izumi Y, Bamba T, Fukusaki E, Hanayama R, Shimada S, Maeda N, Shimomura I.
JCI Insight. 3(8):e99680 (2018) link


2017

High purity isolation and sensitive quantification of extracellular vesicles using affinity to TIM4.
Yoshida T, Ishidome T, Hanayama R.
Curr Protoc Cell Biol. 77:3.45.1-18 (2017) link

Induction of live cell phagocytosis by a specific combination of inflammatory stimuli.
Ishidome T, Yoshida T, Hanayama R.
EBioMedicine. 22:89-99 (2017) link

MiR-21-5p in urinary extracellular vesicles is a novel biomarker of urothelial carcinoma.
Matsuzaki K, Fujita K, Jingushi K, Kawashima A, Ujike T, Nagahara A, Ueda Y, Tanigawa G, Yoshioka I, Ueda K, Hanayama R, Uemura M, Miyagawa Y, Tsujikawa K, Nonomura N.
Oncotarget. 8(15):24668-78 (2017) link


~2016

A novel affinity-based method for the isolation of highly purified extracellular vesicles.
Nakai Y, Yoshida T, Diez D, Miyatake Y, Nishibu T, Imawaka N, Naruse K, Sadamura Y, Hanayama R.
Sci Rep. 6:33935 (2016) link

Mechanisms of lysosomal exocytosis by immune cells.
Song J, Hanayama R.
Chronic Inflammation. Miyasaka M and Takatsu K. eds. (Springer) 369-75 (2016) link

Neuronal exosomes facilitate synaptic pruning by up-regulating complement factors in microglia.
Bahrini I, Song J, Diez D, Hanayama R.
Sci Rep. 5:7989 (2015) link

Autoimmune diseases and the role of MFG-E8.
Hanayama R.
MFG-E8 and Inflammation. Wang P. ed. (Springer) 97-117 (2014) link


Mentored Research

Two-step engulfment of apoptotic cells.
Toda S, Hanayama R, Nagata S.
Mol Cell Biol. 32(1):118-25 (2012) link

The Angelman Syndrome protein Ube3A regulates synapse development by ubiquitinating Arc.
Greer PL*, Hanayama R* (*co-first authors), Bloodgood BL, Mardinly AR, Lipton DM, Flavell SW, Kim TK, Griffith EC, Waldon Z, Maehr R, Ploegh HL, Chowdfury S, Worley PF, Steen J, Greenberg ME.
Cell. 140(5):704-16 (2010) link

Autoimmuniy and the clearance of dead cells.
Nagata S, Hanayama R, Kawane K.
Cell. 140(5):619-30（2010）link

Engulfment of apoptotic cells and its physiological roles.
Hanayama R, Miyanishi M, Yamaguchi H, Suzuki J, Nagata S.
eLS. (Wiley) 165-75（2009）link

Bridge over troubled water: milk fat globule epidermal growth factor 8 promotes human monocyte-derived macrophage clearance of non-blebbing phosphatidylserine-positive target cells.
Witasp E, Uthaisang W, Elenstrom-Magnusson C, Hanayama R, Tanaka M, Nagata S, Orrenius S, Fadeel B.
Cell Death Differ. 14(5):1063-5 (2007) link

Opposite effects of Rho family GTPases on engulfment of apoptotic cells by macrophages.
Nakaya M, Tanaka M, Okabe Y, Hanayama R, Nagata S.
J Biol Chem. 281(13):8836-42 (2006) link

MFG-E8 dependent clearance of apoptotic cells, and autoimmunity caused by its failure.
Hanayama R, Miyasaka K, Nakaya M, Nagata S.
Curr Dir Autoimmun. 9:162-72（2006）link

Impaired involution of mammary glands in the absence of milk fat globule EGF factor 8.
Hanayama R, Nagata S.
Proc Natl Acad Sci USA. 102(46):16886-91 (2005) link

Masking of phosphatidylserine inhibits apoptotic cell engulfment and induces auto-antibody production in mice.
Asano K, Miwa M, Miwa K, Hanayama R, Nagase H, Nagata S, Tanaka M.
J Exp Med. 200(4):459-67 (2004) link

Autoimmune diseases and impaired uptake of apoptotic cells in MFG-E8-deficient mice.
Hanayama R, Tanaka M, Miyasaka K, Aozasa K, Koike M, Uchiyama Y, Nagata S.
Science. 304(5674):1147-50 (2004) link

Expression of MFG-E8 in bone marrow derived immature dendritic cells for engulfment of apoptotic cells.
Miyasaka K, Hanayama R, Tanaka M, Nagata S.
Eur J Immunol. 34(5):1414-22 (2004) link

Expression of developmental endothelial locus-1 in a subset of macrophages for engulfment of apoptotic cells.
Hanayama R, Tanaka M, Miwa K, Nagata S.
J Immunol. 172(6):3876-82. (2004) link

Tethering of apoptotic cells to phagocytes through binding of CD47 to Src homology 2 domain-bearing protein tyrosine phosphatase substrate-1.
Tada K, Tanaka M, Hanayama R, Miwa K, Shinohara A, Iwamatsu A, Nagata S.
J Immunol. 171(11):5718-26. (2003) link

Identification of a factor that links apoptotic cells to phagocytes.
Hanayama R, Tanaka M, Miwa K, Shinohara A, Iwamatsu A, Nagata S.
Nature. 417(6885):182-7. (2002) link

Association of polymorphism in the promoter region of the apolipoprotein E gene with diastolic blood pressure in normotensive Japanese.
Katsuya T, Sato N, Asai T, Fukuda M, Takiuchi S, Fu Y, Hanayama R, Moriguchi A, Rakugi H, Higaki J, Ogihara T.
Hypertens Res. 23(3):271-5. (2000) link

A high expression of heme oxygenase-1 in the liver of LEC rats at the stage of hepatoma: possible implication of induction in uninvolved tissue.
Matsumoto A, Hanayama R, Nakamura M, Suzuki K, Fujii J, Tatsumi H, Taniguchi N.
Free Radic Res. 28(4):383-91. (1998) link